Axion Columns

Ion Exchange Columns

Key Features:

Mobile Phase Compatibility: Compatible with aqueous and most organic solvents
pH Range: Operates efficiently between pH 2 to 8
Pore Size Range: 300 Å to 1000 Å
Particle Size Range: 6μm to 7μm
Column Dimensions: Length: 33 mm to 250 mm and Inner Diameter: 2.1 mm to 10 mm
Surface chemistry: Available with proprietary polyamide as well as crosslinked polyethylenimin
MW ranges for weak Cation and Anion exchange columns: For separation of proteins up to 200000 Da
Functional groups:
For Cation exchange columns: Sulfopropyl
For Anion exchange columns: Carboxymethyl, Quaternary Amine (fully quaternized PEI)

Note:

Cation exchange columns are based for analysis of enzymes, proteins, catecholamines, peptides, hemoglobin variants, glycosylated hemoglobins, and crystallins.
We also offer Q-300 column which has a support whose ionization has no pH dependence.
All of our strong Cation exchange column supports have constant ionization above pH 3.

Specifications:

AX300 (Weak Anion Exchanger)

AX300 support is a crosslinked polyethyleneimine phase on 6-7µ, 300Å, silica. Selectivity can be altered by mobile phase composition, and pH affects ionization of both the support and the solute. AX-300 permits analysis of proteins up to molecular weights of 200,000 daltons. AX300 has excellent recoveries and loading capacity e.g., 22mg ovalbumin can be loaded onto a 250x4.6 column with no overloading effects.

CM300 (Weak Cation Exchanger)

CM300 support has a polyamide coating containing carboxymethyl groups and is bonded to a 6-7µ, 300Å, silica. Selectivity can be altered by mobile phase salt composition, and pH affects ionization of both the support and the solute. CM-300 permits analysis of proteins up to molecular weights of 200,000 daltons and exhibits excellent recoveries as well as high loading capacity.

S300 (Strong Cation Exchanger)

S300 support has a polyamide coating containing sulfopropyl functional groups, bonded to a 6-7µ, 300Å, silica. Ionization of the functional groups on this bonded phase is constant above pH 3. S-300 columns are a good choice for the separation of basic proteins.

S1000 (Strong Cation Exchanger)

S1000 support has a polyamide coating containing sulfopropyl functional groups bonded to a 7µ, 1000Å, silica. Ionization of just the solute is affected by pH above pH 3. S-1000 columns are excellent columns for the fast analysis of glycosylated hemoglobin. The support has a polyamide coating containing sulfopropyl functional groups bonded to a 7µ, 100Å, silica. Ionization of just the solute affected by pH above pH3. S-1000 columns are excellent columns for the analysis of glycosylated hemoglobin

Q300 (Strong Anion Exchanger)

Q300 support has fully quaternized, crosslinked polyethyleneimine phase on 6-7µ, 300Å silica. Ionization of this support has no pH dependence. Q-300 columns are excellent columns for the rapid separation of proteins and enzymes.