IonoSEC GPC Columns

IonoSEC columns are designed and manufactured for the analysis and purification of proteins, peptides, polymers, basic and acidic molecules, as well as pharmaceutical compounds These columns are based on pure, highly spherical, porous, and non-porous silica supports Designed to deliver excellent resolution, stability, and reproducibility


High-Resolution Size Exclusion Chromatography

  • Available in 7 pore sizes (50Å–4000Å) & 5–10µm particles
  • Built with glycerol-bonded size exclusion & spherical silica chromatography chemistry
  • Broad column dimensions: 1–30mmI.D., 50–300mm length
  • Ideal for 0.5kDa to 10,000 kDa molecular weight range
  • USP L20 & L33 compliant for regulatory confidence
  • 100Å – 500Å: Fast analysis of proteins, carbohydrates, nucleic acids, and other water-soluble polymers
  • 1000Å & 4000Å: Great for large polymer separation

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Specifications:


IonoSEC GPC

IonoSEC GPC PEPTIDE is a glycerol bonded size exclusion support on a 5μ, 50Å spherical silica which can resolve small peptides (MW 0.8-30 kD). This support can resolve peptides which have at least a two-fold MW difference. Mobile phase optimization may be necessary due to the high variability in solubility, charge, and hydrophobicity of peptides. The exclusion limit for IonoSEC GPC Peptide makes it an effective "desalting column" for processing protein samples.


IonoSEC GPC-100


IonoSEC GPC-100 is a glycerol bonded size exclusion support on 5μ, 100Å spherical silica designed for the rapid analysis of proteins, carbohydrates, nucleic acids, and other water-soluble polymers. This support is appropriate for globular molecules with MW ranges from 5-160 kD and linear molecules with MW ranges from 0.5-25 kD.


IonoSEC GPC-300


IonoSEC GPC-300 is a glycerol bonded size exclusion support on 5μ, 300Å spherical silica designed for the rapid analysis of proteins, carbohydrates, nucleic acids, and other water-soluble polymers. This support is appropriate for globular molecules with MW ranges from 10-1000 kD and linear molecules with MW ranges from 2-100 kD.


IonoSEC GPC-500


IonoSEC GPC-500 is a glycerol bonded size exclusion support on 7μ, 500Å spherical silica designed for the rapid analysis of proteins, carbohydrates, nucleic acids, and other water-soluble polymers. This support is appropriate for globular molecules with MW ranges from 40-2000 kD and linear molecules with MW ranges from 10-350 kD.


IonoSEC GPC-1000


IonoSEC GPC-1000 is a glycerol bonded size exclusion support on a 7μ, 1000Å spherical silica designed to effectively analyze polymers by size. This support has minimal interaction with anionic and neutral water-soluble polymers and is most frequently used with linear molecules possessing MW ranges from 40-1000 kD. It also allows for the separation of globular molecules such as protein multimers with MW ranges from 400-10,000 kD.


IonoSEC GPC-Linear


IonoSEC GPC Linear is a glycerol bonded size exclusion support on a 7μ mixed pore distribution (100/1000 Å) spherical silica allowing analysis of samples with a broad range of MWs. This support has minimal interaction with anionic and neutral water-soluble polymers and denatured protein possessing MW ranges from 1-1000 kDa.


IonoSEC GPC-4000


IonoSEC GPC-4000 is a glycerol bonded size exclusion support on a 10μ, 4000Å spherical silica designed to effectively analyze linear polymers by size. This support has minimal interaction with anionic and neutral water-soluble polymers and is most frequently used with samples containing linear molecules having MW >10,000 kD.